Staining with T1 thioflavin: It is a protein that in solution is not fluorescent. When light is rotated, like heat, it dissipates. When it binds to the amyloid protein, it cannot rotate and then fluoresces. Amyloid changes its conformation and becomes more resistant to proteases.
Thank you very much for your great research effort Karl C. I am also very grateful to all the contributions of Dr. David Nixon.
I would like to make the following contribution, Karl:
Amyloidosis is a heterogeneous group of diseases that consists of the abnormal folding of a precursor protein that ends up being deposited in the form of fibr…