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Let’s do some basic math and systems biology for HaJo, since he seems to prefer 'simple' questions over complex reality:
The Timeline of Humoral Response:
Data from Fooksman et al. (2010) shows that while short-lived plasma cells appear early, the high-affinity late PCs from germinal centers take time to coalesce–usually around day 6 and beyond (in mice). According to Schultheiß et al. (2021), we see a clear maturation trajectory. Plasmablasts (PBs) expand significantly following exposure.
Schultheiß C, Paschold L, Willscher E, Simnica D, Wöstemeier A, Muscate F, Wass M, Eisenmann S, et al. Maturation trajectories and transcriptional landscape of plasmablasts and autoreactive B cells in COVID-19. iScience. 2021 Nov 19;24(11):103325. doi: 10.1016/j.isci.2021.103325. Epub 2021 Oct 23. PMID: 34723157; PMCID: PMC8536484.
See also: Khodadadi L, Cheng Q, Radbruch A, Hiepe F. The Maintenance of Memory Plasma Cells. Front Immunol. 2019 Apr 5;10:721. doi: 10.3389/fimmu.2019.00721. PMID: 31024553; PMCID: PMC6464033.
Fooksman DR, Schwickert TA, Victora GD, Dustin ML, Nussenzweig MC, Skokos D. Development and migration of plasma cells in the mouse lymph node. Immunity. 2010 Jul 23;33(1):118-27. doi: 10.1016/j.immuni.2010.06.015. Epub 2010 Jul 8. PMID: 20619695; PMCID: PMC2952879.
The Mechanistic Delay: Let’s look at the actual cascade:
Uptake & Escape: It takes 1–2 days for LNPs to distribute, to transfect cells and for a sufficient amount of modRNA (approx. 3–15% escape rate; unknown rate, unknown ratios of unknown cell types) to get translated.
Translation: It takes another ~3 days until enough antigen is expressed to effectively recruit and prime B-lymphocytes (+ a lot of fragmented and frameshifted garbage proteins).
Maturation: Add the 5–8 days required for B-cell maturation and the production of neutralizing antibodies.
The Result: 1 + 3 + 8 = 12 days. Looky, looky, HaJo: That is exactly the 'Day 12' mark from the Polack et al. study where the curves start to diverge and PCR positivity in the vaccinated group drops. It’s not a mystery; it’s basic immunological kinetics.
However, isn’t it somehow funny that you rely on one of the most fradulent studies where the whole data was flawed and faked? So who is the submarine? 🤔
The neutralizing antibody-effect begins exactly when the humoral arm of the immune system has finished its sequence. This occurs regardless of your obsession with DNA “scams” or “Process 1 vs. 2”–it is a matter of biological lead time not of structure and LNP-physicochemistry.
Maybe if you stopped hunting for “honeypots” and started reading papers on recruitment and maturation of B-lymphocytes, you’d realize that your “simple question” was answered by science a long time ago. But I guess that doesn't fit the “submarine” narrative, does it?
And before asking why: Ask what: What is the basis of the RT-PCR after quaccination? At what cycle?
